Hello!
Sorry but I can't speak French!
I thought to share my SIM (structured illumination microscope) project on my Zeiss Universal on this interesting forum.
Structured illumination allows to record confocal-like images of fluorescent samples. Due to the slow acquisition rate of my system (10-15 seconds for each slice), only still, fixed samples can be recorded.
Every part, like mechanical device for grid rotation, stage, camera, focusing knob, is controlled by two Arduino UNO rev3 and an Adafruit TFT 2,8" touch screen.
Here you can find a ppt of the project: https://lookaside.fbsbx.com/file/Zeiss%20Universal%20SIM.ppsx?token=AWxdNNKB-HE_0cIR25unVl0ggoG0TtAp-OGIFgS9pJM41XnxOTiY2WE4nenmj-xBNq5mAVrloCfG6KvFleccIIhQpbk_QxNAwKHQJ2_lii2qAyhGDNqw355NrKpxAnh1iQ8gHX755Pq0fbP8QpZ8VbleAxvcl327g-vFa8Cre9MiLpXsp98XuouBZEz9nq5T5LwK_pTNgB9_jTP7hYfZGAOA
Here the code for the SIM controller:
And here the code for the mechanical stage control:
Testing and fine tuning the system is still in progress, but the first results seem to go to the right direction. The contribute of out of focus parts is eliminated: